Web凝胶过滤柱层析 介质:Sepharose 4 FF 超滤浓缩到适当体积 按照平衡柱床、丄样、洗脱程序层析 ①具有准确的转录后修饰功能 ,表达的糖基化药 物蛋白在分子结构、 理化特性和生物学功能方面最 接近于天然蛋白分子 ②具有产物胞外分泌功 能 ,便于下游产物分离纯化; ③具有重组基因的高效 扩增和表达能力; ④具有贴壁生长特性 ,且有较高的 耐受剪切力和渗透压能 … Web填料类型: IMAC Sepharose:固定化金属离子亲和层析填料,基架已经活化好了, 可用于直接耦合金属离子。 Ni Sepharose:已经耦合了Ni离子,可以直接用于纯化His蛋白,载量极 高,脱落极低。 Chelating Sepharose:亚氨双乙酸耦合的填料,可用结合不同的金属离 子,脱落低。 30 20 10 20 40 60 80 100 Protein added (mg/1 ml column) Ready in less than 15 …
凝胶过滤填料的分离范围表-北京更新生物科技有限公司
WebSP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. Change Product. ID. Product Name. 17072905. SP … WebDescription The base matrix, Sepharose 4 Fast Flow, is a cross-linked, 4% agarose derivative with excellent kinetics, making them ideal for process scale applications, particularly during initial capture and intermediate stages of a separation … o\u0027bannon soccer fields
HiTrap IMAC Sepharose FF Cytiva
Web1. Equilibrate all material to room temperature. 2. Sepharose Fast Flow ion exchange media are supplied preswollen in 20% ethanol. Decant the ethanol solution and replace it with packing buffer to a total volume of 32.5 ml (75% settled gel: 25% buffer). 3. Degas the slurry under vacuum. 2. Assembling the column 1. WebHiTrap DEAE FF is prepacked with DEAE Sepharose Fast Flow, and is a weak anion exchanger for small-scale protein purifications as well as screening of binding and … WebAs member of the BioProcess media range, SP Sepharose ® Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support. … o\u0027bannons college station