WebA phosphoenolpyruvate (PEP) phosphatase was purified to homogeneity from germinating mung beans (Vigna radiata). It was found to be a tetrameric protein (molecular mass 240,000 daltons) made up of apparently identical subunits (subunit molec-ular mass 60,000 daltons). It was free from bound nucleotides. It did not show pyruvate kinase activity. WebAcid phosphatase isoenzyme (AcP-II) from leaves of germinating seeds of vigna radiata (mung beans) was partially purified by CM-Cellulose chromatoghraphy, gelfiltration on Ultrogel AcA 44 and Con A-Sepharose affinity chromatoghraphy. The specific
Association of Phosphoenolpyruvate Phosphatase Activity with …
WebThis chapter will discuss some practical aspects of alkaline phosphatase assays. While the remarks are based predominantly on experience gained with human blood serum, many of the points raised are also applicable to the assay of alkaline phosphatases from other sources. Keywords Alkaline Phosphatase Alkaline Phosphatase Activity WebPEP phosphatase activity profile (Fig. 1C) and the associated protein band quantified by laser densitometric scanning of the Coomassie blue-stained native gel (Fig. 1A) indicated that the final mung bean PEP phosphatase preparation was no more than 6% pure. Given the final specific activity of 1.4 units/mg, a specific activity no less than 23 ... lee smith the tablet
Combined use of phosphate solubilizing bacteria and
WebMung bean acid phosphatase reacted immunologically with primary antibodies against Solanum tuberosum acid phosphatase, while polymerase chain reaction (PCR) analyses … WebAug 17, 2024 · Mung beans have several other biological activities, such as angiotensin I-converting enzyme (ACE) inhibition, reducing lipid accumulation, and lowering inflammation. ACE inhibition plays a crucial role in reducing blood pressure . Mung beans contain polyphenols, vitexin, isovitexin, flavonoids, gallic acid, and organic acids. WebNov 16, 2012 · In the present study, acid phosphatase enzyme was isolated and partially purified from germinated local mung bean seeds. The sequential partial purification … how to file for llc