Web16 dec. 2024 · Serum deprivation potently induces ciliogenesis by increasing the proportion of cells in Go phase and removal of secreted factors that inhibits ciliogenesis 12, 13. In addition, mitochondria fuse... Web30 sep. 2015 · Cilia incidence significantly increased in cobblestone-treated HMEC-1s compared to confluent HMEC-1s, for low and high serum conditions (Fig. 5 p < 0.001 …
Low fluid shear stress promoted ciliogenesis via Dvl2 in …
Web12 dec. 2013 · We seeded equal amounts of Atg5+/+ and atg5−/− MEFs on cell dishes for 8–12 h to reach 90% confluency and immediately treated these cells in low-serum … WebIn standard NIH3T3 culture medium, without enforced induction of cilia formation, the percentage of ciliated cells differ between ~3% and ~18% ( Figure 1 A,B). Even an … creepy scary halloween door decorations
Commentary: Self-eating to remove cilia roadblock - PMC
WebWe seeded equal amounts of Atg5+/+ and atg5−/− MEFs on cell dishes for 8–12 h to reach 90% confluency and immediately treated these cells in low-serum (0.5% FBS) medium … To identify the component in serum that can induce cilia disassembly, we set up a cilia disassembly assay with cultured human retinal pigment epithelial (RPE-1) cells13. Briefly, we serum-starved RPE-1 cells for 48 h to induce cilia formation in the majority of cells. Ciliated cells were then re-stimulated with serum … Meer weergeven LPA acts through a family of high-affinity G-protein-coupled receptors, LPAR1-641, only LPAR1 was highly expressed in RPE-1 cells … Meer weergeven We next explored the physiological role of LPA-induced cilia disassembly in mice. Lpar1 is enriched in mouse VZ/SVZ51. LPA and its major producing enzyme autotaxin … Meer weergeven To decipher the mechanism underlying how LPA-LPAR pathway promotes cilia disassembly, we performed RNA-Seq to analyze the transcriptome changes in control and LPAR1-depleted cells with or without LPA … Meer weergeven Next, we evaluated whether Lpar1 depletion influences the proliferation and self-renewal of NPCs. VZ/SVZ cells in Lpar1−/− … Meer weergeven Web1 jan. 2009 · In 1979 Tucker showed that 80–90% of 3T3 fibroblasts form cilia after growing for 48 h in low serum medium, at a confluence of 80–100% (Tucker et al., 1979a). For the cell lines discussed here, the time of incubation in serum-free medium required for the induction of cilia varies, and should be adjusted for each cell type individually. buck stove ventless gas stoves