Jcrb9068
Web5 nov 2024 · HEK293 cells were purchased from JCRB Cell Bank (JCRB Cell Bank Cat No. JCRB9068, RRID: CVCL_0045, Osaka, Japan), who stated authentication. The cells were maintained in Dulbecco's modified Eagle's medium (DMEM; Nacalai Tesque, Kyoto, Japan) supplemented with 10% fetal bovine serum, 100 U ml −1 of penicillin, and 100 μg ml −1 … Web6 giu 2024 · Medium. Eagle's minimam essential medium with non-essential amino acids and 5% calf serum. Temperature. 37 C. Cell Density at Seeding. 4 x 10^4 cells/ml. …
Jcrb9068
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Web18 feb 2016 · HEK293 (JCRB9068), HeLa (JCRB9004) and Huh7 (JCRB0403) were from Japanese Collection of Research Bioresources. RNA interference siRNA transfection was described. 25 Anti-p63 siRNA (p63si) consisted of IMXRU (sense RNA, 5′-ggacguauuccacugaacutt-3′; antisense RNA, 5′-aguucaguggaauacgucctt-3′) and CUBCP … Web3 giu 2024 · The HEK293 cells (JCRB9068, Lot 061496) were purchased from the National Institute of Health Sciences (Kanagawa, Japan). Antibodies The mouse mAb S16 was …
Web3 dic 2024 · The ACE2 gene was inserted into Adeno-X Adenoviral System 3, resulting in pAdX-ACE2. Ad-ACE2 was propagated in HEK293 cells (JCRB9068, JCRB Cell Bank). Beta-galactosidase (LacZ)-expressing Ad vectors were purchased from Vector Biolabs. The vector particle (VP) titer was determined by spectrophotometry. 21. ACE2-expressing … Web3 dic 2024 · To overcome this shortcoming, a new system that enables ssDNA-based promoter replacement is required. In this study, we created a CRISPR-ssDNA system …
Web3 giu 2024 · The HEK293 cells (JCRB9068, Lot 061496) were purchased from the National Institute of Health Sciences (Kanagawa, Japan). Antibodies. The mouse mAb S16 was produced by Sugawara et al. 27. Web13 dic 2024 · JCRB9127) and HEK293 (JCRB9068), were obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). The cells were cultured …
Web1 mar 2024 · Section snippets Cell cultures and transfection. COS-7 (African green monkey kidney-derived cell line; Cell #. JCRB9127), HEK293 (human embryo kidney-derived cell line; JCRB9068), HeLa (human cervix epithelioid carcinoma cell line; JCRB9004), Hep G2 (human hepatocellular carcinoma cell line; JCRB1054), and MCF-7 (human breast …
WebHEK293 (HSRRB, Osaka, Japan; No. JCRB9068) cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco/Invitrogen) supplemented with 10% Fetal Bovine Serum … cygames 4gamer 出禁Web28 gen 2024 · HepG2 (JCRB1054), HEK293 (JCRB9068), and HeLa (JCRB9004), which were obtained from the Japanese Collection of Research Bioresources Cell Bank, McA-RH7777 (ATCC CRL-1601) obtained from American ... cygames 4gamerWebrespectively) were propagated in HEK293 cells (JCRB9068, JCRB Cell Bank). LacZ-expressing Ad vectors were purchased from Vector Biolabs. The vector particle (VP) titer was determined by using a spectrophotometric method (Maizel Jr et al., 1968). Viral titration of SARS-CoV-2 Viral titers were measured by median tissue culture infectious dose ... cygames 10th anniversaryWebkidney cells) (JCRB9068, Health Science Research Resources Bank, Osaka, Japan) were maintained in Dulbecco’s modified Eagle’s medium, supplemented with 10% heat-inactivated fetal bovine serum ... cygames budget cutsWeb27 nov 2015 · Many genes and signaling pathways have been found to be involved in cellular senescence program. In the present study, we have identified 16 senescence-associated genes by differential proteomic ... cygames historyWeb7 dic 2024 · The HEK293 (JCRB9068) cells using in retrovirus production were purchased from the Health Science Research Resources Bank (Japan Health Sciences Foundation, … cygames incomeWeb7 dic 2024 · The HEK293 (JCRB9068) cells using in retrovirus production were purchased from the Health Science Research Resources Bank (Japan Health Sciences Foundation, Osaka, Japan). cygames ai